Glycerin fraction is a by-product in a process of higher fatty acid methyl esters production. Apart from pure glycerin, it also contains admixtures of methyl alcohol, mono- and diglycerides, free fatty acids, colouring substances and soaps. Glycerin fraction is used as well as an energy source in combustion process or microbiological production of biogas. It is used as a supplement to fodder and after refining it becomes a valuable raw material for cosmetic, food and chemical industry.